Formation from Trypsin and Modified Soybean Trypsin Inhibitor of a Complex Which upon Kinetic Control Dissociation Yields Trypsin and Virgin Inhibitor
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Formation from trypsin and modified soybean trypsin inhibitor of a complex which upon kinetic control dissociation yields trypsin and virgin inhibitor.
It is shown that kinetic control dissociation of a complex prepared by mixing of equimolar quantities of trypsin and of modified (reactive site Arg (64)-Ile bond cleaved) soybean trypsin inhibitor (Kunitz) yields trypsin and predominantly ( >95%) virgin (reactive site intact) inhibitor. I f less than equimolar quantities of trypsin are used the fraction of inhibitor molecules in which the bond ...
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Five trypsin and cu-chymotrypsin inhibitors which have low molecular weights (ranging from 6800 to 8600) and are present in soybean seeds of the Tracy variety have been isolated and purified, and single crystals which give x-ray diffraction data beyond 3-A spacings have been obtained from one of them. The trypsin inhibitor crystallizes in a monoclinic unit cell of symmetry P2, and dimensions a ...
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Methods are described for the isolation and crystallization of trypsinogen, trypsin, a substance which inhibits trypsin, and an inhibitor-trypsin compound. Analyses and some of the properties of these compounds are given.
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1970
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)63202-8