Formation from Trypsin and Modified Soybean Trypsin Inhibitor of a Complex Which upon Kinetic Control Dissociation Yields Trypsin and Virgin Inhibitor

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Formation from trypsin and modified soybean trypsin inhibitor of a complex which upon kinetic control dissociation yields trypsin and virgin inhibitor.

It is shown that kinetic control dissociation of a complex prepared by mixing of equimolar quantities of trypsin and of modified (reactive site Arg (64)-Ile bond cleaved) soybean trypsin inhibitor (Kunitz) yields trypsin and predominantly ( >95%) virgin (reactive site intact) inhibitor. I f less than equimolar quantities of trypsin are used the fraction of inhibitor molecules in which the bond ...

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Five trypsin and cu-chymotrypsin inhibitors which have low molecular weights (ranging from 6800 to 8600) and are present in soybean seeds of the Tracy variety have been isolated and purified, and single crystals which give x-ray diffraction data beyond 3-A spacings have been obtained from one of them. The trypsin inhibitor crystallizes in a monoclinic unit cell of symmetry P2, and dimensions a ...

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A study has been made of the general properties of crystalline soybean trypsin inhibitor. The soy inhibitor is a stable protein of the globulin type of a molecular weight of about 24,000. Its isoelectric point is at pH 4.5. It inhibits the proteolytic action approximately of an equal weight of crystalline trypsin by combining with trypsin to form a stable compound. Chymotrypsin is only slightly...

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Isolation and Characterization of Trypsin Inhibitors (Kunitz Soybean Trypsin Inhibitor, Bowman-birk Inhibitor) in Soybean

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Isolation from Beef Pancreas of Crystalline Trypsinogen, Trypsin, a Trypsin Inhibitor, and an Inhibitor-trypsin Compound

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ژورنال

عنوان ژورنال: Journal of Biological Chemistry

سال: 1970

ISSN: 0021-9258

DOI: 10.1016/s0021-9258(18)63202-8